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KMID : 0355519900140010019
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Korean Journal of Oral Biology
1990 Volume.14 No. 1 p.19 ~ p.30
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SYSTEMIC ABSORPTION, DISTRIBUTION AND ACUTE TOXICITY OF FORMALDEHYDE IN PULPOTOMY TREATMENT USING FORMOCRESOL
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Abstract
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Formaldehyde that is active constituent in formocresol used in pulpotomy is known as toxic agent that causes systemic toxicity.
To determine the fate of the 14 C-formaldehyde which was absorbed following its application to pulpotomy sites, by-pass of posterior facial vein and cannulation of external carotid artery were established with 20G-polyethylene tube. Blood samples (0.2ml) were collected in glass syringe before the pulpotomy and then at 0, 3, 5, 10, 20, 30, 45, 60, 90 and 120 minutes thereafter. Plasma was separated after centrifugation at 3000 x g for 15 min and aliquots of plasma were counted by liquid scintillation counter.
After liver, kidney, lung, heart, spleen, muscle, and alveolar socket were removed and solubilized with tissue solubilizer, the radioactivities in solutions were counted by liquid scintillation counter.
The acute toxicity of formocresol was determined by BUN, GOT and OCT assay in blood samples collected at 0, 2, 4, 6, 9 and 12 hours by means of cardiac puncture.
The results were as follows;
1. The radioactivity of 14 C-formaldehyde reached maximal value at 3-5 minutes and decreased there-after.
2. About 8% of 14 C-formaldehyde placed in the pulpotomy site was actually absorbed systemically until 2 hours.
3. About 50% of 14 C-formaldehyde absorbed actually was distributed within 20 minutes.
4. The radioactivity of organ per gram was highest in the spleen and lowest in the muscle.
5. Total radioactivity of organ was highest in the liver and lowest in the heart.
6. There were no significant differences between control and experimental groups in BUN, GOT, and OCT assay.
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